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8 - Evaluation of AntiHIV test results of Şişli Etfal Hospital from 2000 to 2006
Banu Bayraktar, Emin Bulut, Hatice Erdoğan
Objective: The aim of this study is to determine positive results rate of the AntiHIV Enzyme linked immunosorbent assay] ELISA) and how many of them could be confirmed by western blot (WB) from 03.04.2000 to 24.10.2006 in our laboratory.
Study Design: We analysed our laboratory AntiHIV test reports retrospectively. 82304 serum samples were tested for HIV infection by different ELISA kits by using one at a time, namely HIV 1-2 Innogenetics, Vironostika HIV Uni-Form II Ag/Ab (Biomerieux), AxSYM HIV-11210* [MEIA (Micropartical Enzyme Immuno Assay) Abbott], and Architect HIV Ag/Ab Combo (Chemiflex technology, Abbott). Positive samples were sent to the Istanbul Health Authority Epidemiology Department for WB confirmation testing and the results are reported here.
Results: During the 6 years period 82304 serum samples were tested for AntiHIV antibodies with ELISA. 80 ELISA positive serum samples were further tested with WB for confirmation. 50 out of 80 samples were found negative and 30 were confirmed as positive.
Conclusions: Although ELISA is a reliable test for HIV infection screening, positive results must be confirmed with WB.
Keywords: AntiHIV ELISA, western blot
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