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6 - The identification and research of resistance to antibiotics of enterococcus spesies isolated from clinical specimens
Birsen Durmaz Çetin, Nuran Özcan, Mehtap Oktar, Hatice Hasman, Hülya Kımıl
Objective: Identification and research of resistance to antibiotics of 88 enterococcus species isolated from wound and urine specimens in Şişli Etfal Training and Research Hospital Microbiology Laboratory is aimed.
Material and Methods: The species that are sent to our laboratory between the dates of 1999 and 2002 were identified by growth in bile esculin agar and 6.5% NaCl, hemolysis characteristics in sheep blood agar, catalase test, Gram stain and API 20 Strep (Bio-Merioux, France) tests. For the search of hetalactamase activity, Beta-lactamase (nitrocefin) Identification Sticks (Oxoid) were used. The antibiotic sensitivities of the enterococcus species isolated from wound and urine specimens were found by disc diffusion method to vancomycin, teicoplanin, penicilin, am- picillin, ciprofloxacin, erythromycin, tetracycline, nitrofurantoin according to antibiotic groups recommended by NCCLS. High level gentamycin (120 pg) disc was used to show high level aminoglycoside resistance.
Results: 28 of 88 enterococcus species (32%) were isolated from wounds and abscesses, 60 (68%) from urine specimens. It is found that 54 (61%) of them were Efaecalis, 27 (30%) E.faecium, four (4.5%) E.avium, two (2.2%) E.durans and one (1.1%) E.raffinosum. Resistance ratio to antibiotics were 56% to penicillin, 52% to ampicillin, 68% to ciprofloxacin, 61% to erythromycin, 54% to tetracycline and 77% to nitrofurantoin. High level genta- micin resistance was found in 26 of88 (29%) enterococcus species. There werent any resistant species against vancomycin or teicoplanin and no beta-lactamase enzyme in enterococci isolated in our study.
Conclusion: Identification and antibiotic sensitivity tests must be searched because resistance pattern change according to species in treatment regimens in enterococci infections.
Keywords: Enterococcus, antibiotic resistance, beta-lactamase.
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