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9 - Antibiotic resistance of staphyloccocal strains isolated from wound specimens
Birsen Durmaz Çetin, Nuran Özcan, Mehtap Oktar, Alper Gündüz, Mustafa Gül
Objective: To investigate the antibiotic resistance of 1080 staphyloccocal strains isolated from wound specimens sent to the microbiology laboratory by different clinics at Sisli Etfal Training and Research hospital between 1999-2002.
Study Design: The identification of bacteria was performed by colony morfology, growth and gram staining features, catatase and coagulase tests. The antibiotic resistance of these strains to methicillin, sefazolin, erythromycin, tetracycline, gentamicin, amikacin, ciprofloxacin, TMP/SXT was investigated according to NCCLS criteria by Kirby-Bauer disc diffusion method. The susceptibility to Fusidic acid was performed according to the criteria of Comité de Lantibiogramme de la Société Française de Microbiologie.
Results: Out of the isolated staphyloccocal strains 670 (62%) were S. aureus and 410 (38%) were coagulase negative staphylococci. Among S.aureus strains 260 (38%) and 220 (53%) of coagulase negative staphyloccocal strains were methicillin resistant.
The antibiotic resistance rate to the tested antibiotics was higher among the methicillin resistant strains. Vancomycin resistance was not found. The most active antibiotics against methicillin resistant strains were TMP/SMX, fusidik Asit, amikasin, ciprofloxacin, gentamicin.
Conclusions: It seems that in staphyloccocal infections developing in hospitals, besides methicillin resistance, , multiple drug resistance continues to be a serious problem in our hospital.
Keywords: Staphylococci, antibiotic resistance, wound specimen
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