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7 - Determination of candida species and their antifungal susceptibilities isolated from inpatients
Alper Togay, Banu Bayraktar, Dilek Yıldız Sevgi, Emin Bulut
Objective: Fungi have emerged as major causes of human disease recently, especially among the immunocompromised patients and those hospitalized with serious underlying diseases. The aim of this study was to identify Candida species isolated from different cultures and determine their antifungal susceptibilities retrospectively and define of value of using commercial system.
Material and Method: Test results of yeasts that were isolated from 45 blood, 10 urine, three bronchoalveolar lavage and one catheter samples of the patients during the period January 2011-December 2013 in our laboratory were evaluated.The yeasts were identifed by germ tube test, CHROMagar Candida (CHROMagar, Paris, France) and AuxaColor (Bio-Rad SDP, Paris, France) commercial identifcation system. The antifungal susceptibility tests were performed using Fungitest (Bio-Rad SDP, Paris, France) commercial system.
Results: The distribution of 71 candida species was as follows: 28 Candida albicans (39%), 13 Candida parapsilosis (18.3%), 11 Candida glabrata (15.5%), 10 Candida tropicalis (14.1%), four Candida krusei (5.6%), three Candida lusitaniae (4.2%), one Candida kefyr (1.4%) and one Candida dubliniensis (1.4%). A intermediate C.parapsilosis strain to Amfoterisin B and a resistant C.tropicalis strain to Amfoterisin are evaluated using E-test and found resistant with a minimum inhibitor concentration (MIC) value of 1.5 µg/ml for both of them. The MIC rates of two out of three fluconazole (FLU) intermediate and one resistant C.albicans strains were evaluated using E-test and all three strains were found resistant with a MIC value of 256 µg/ml. The MIC of a FLU intermediate C.albicans strain to FLU was 32 µg/ml. The MIC of a C.tropicalis strain that was FLU resistant with Fungitest was 0,38 µg/ml and was evaluated as susceptible.
Conclusion: In our study, it is important knowledge of species and antifungal susceptibility test results for control of fungal infections and plan of treatment. We used Auxacolor and Fungitest in our study. And this tests were easy to use and to evaluate. It should be carefully evaluated of the Fungitest results which dont accord with the actual guidelines. It would be appropriate to confirm intermediate and resistant results using reference methods.
Keywords: Antifungal susceptibility, Candida species, hospital infection
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